Features of post-radiation disturbances in the accumulation of calcium ions in intracellular platelet depots at different times of the post-radiation period

Calcium ions are an important intracellular regulator; increase in their concentration the cytoplasm plays a key role determining functional activity of platelets associated with platelet aggregation and release reaction. The main way to reduce free calcium is activation Ca2+-ATPases depositing structures plasma membrane, which cytoplasmic content due its reuptake depot (resequestration) Ca2+ from cell. Platelets express two Ca2+-ATPase isoforms different depositor (SERCA2b SERCA3) membrane (PMCA1b 4b). SERCA2b isoform, dense tubular system, inhibited by thapsigargin. SERCA3 isoform present acidic organelles has low sensitivity thapsigargin, but high tBHQ (2,5-di-(tert-butyl)-1,4-hydroquinone). When stimulated micromolar concentrations thapsigargin ionomycin, cause complete depletion depots system acid organelles, differences levels accumulated norm post-radiation period found calcium-free medium: 1.4 times noted on 3rd day. In platelets, under action nmolar inhibit SER-CA2b systems, change deposited irradiated animals was revealed Under conditions inhibition medium day after irradiation at dose 1 Gy, more intense also compared control. On 10th, 30th, 90th day, there were no statistically significant these indicators. calcium-containing medium, intake outside into observed depot-controlled mechanisms period, especially 10th days.